Training: Annotations for pathologists

Metadata table functions

Main metadata table supports the following operations.

• To increase the size of any column, click and drag a separator in the column header, to the right
• To decrease the size of any column, click and drag a separator in the column header, to the left
• To show or hide any of the columns

• Open the columns menu from the right hand side
• De-select all
• Select only the columns you wish to have displayed
• Alternatively to scrolling the list, you can use the quick-filter to find the columns you want to select

• To move columns around, simply click and drag the column header to the place where you wish the column to be shown

Find slides using global filter

• To filter the main metadata table and find only the relevant slides, type in your search in the global search box at top-left
• Example: Find all IF slides

• In the global search box, enter: IF

• Example: Find a single slide, with filename 'b648487_1'

• Copy the text: b648487_1
• In the global search box, enter: b648487_1

Find slides using column filter

• To filter the main metadata table and find only the relevant slides, click on the column hamburger menu,
• Click on the filter icon
• Using column-dedicated filters, find relevant slides
• Example: Find all IF slides

• Click on the hamburger menu in the header of the 'Method' column
• Click on the filter icon
• De-select all, by unselecting the '(Select All)' option
• Select only the 'IF' option

• Example: Find all IF slides (alternative method)

• Click on the Filters menu on the right-hand side
• Expand the 'Method' filter
• De-select all, by unselecting the '(Select All)' option
• Select only the 'IF' option

Sort slides

• To sort the main metadata table click on the header of the column you wish to sort by
• You can click on the header multiple times. Each click will perform a different action

• First click will sort the table in an ascending order
• Second click will sort the table in a descending order
• Third click will reset the sorting to default

Group your search results

• To group slides by specific metadata columns

• Simply drag a column header into the first row, where it's written "Drag here to set row groups"
• You can drag multiple column headers one after another, to group by several criteria
• Expand a group in table by clicking on the arrow before the entry
• Click on the 'X' in the grouping category to remove the grouping of the entries

Edit a metadata table entry

• Double click in the field you wish to change, and either select the entry from the drop-down options, or enter the text you wish to add
• Alternatively, you can use copy-and-paste feature to copy and paste values from and into fields you wish to edit
• Alternatively, you can drag the bottom-right square of a field up or down to edit those cells with the value of the field which is being dragged
• Example: Enter a comment

• Double-click on the 'Comment' field of a slide you wish to edit
• Type a comment, e.g. 'Training image'

Find the admin and ask for access

You can search through and browse all slides available in the CP Portal

• Open the Browse screen, by clicking on the 'Browse' button on the top-right
• Use column filters, or filters on the right hand side to find slides you are interested in

Find the admin and ask for access

• In the Project list (when the list is grouped by project), click on the user symbol next to the project name
• Note the e-mail address of the Admin user. You can write them to get access to that project

Open slide

To view the full image in the image viewer

• Double-click on the thumbnail of a slide. This will open the image in the image viewer
• Zoom in and out of the image using your mouse wheel
• Alternatively, you can control the zoom level by clicking on the magnifications at the bottom-right of the image viewer (e.g. 20X, 40X)
• Rotate the slide using SHIFT+ALT on your keyboard and the mouse pointer
• Pan the image by clicking the left mouse button and moving around
• To quickly navigate to different parts of the image

• Click on the double-left arrow in the top-right of the image viewer
• Drag the red rectangle to where you want to jump on the image

Pixelization (pixel-smoothing) setting

• To turn pixel-smoothing (pixel view) on or off
• Click on the pixelize button at the bottom bar in the image viewer to turn pixel-view on (pixel-smoothing off).
• Click on the pixelize button again at the bottom bar in the image viewer to turn pixel-view off (pixel-smoothing on).

• Note: Changing the pixelization view will be persisted between the sessions

Multiview

• While viewing an image in the image viewer click on the 'Show slide list' button
• Right-click on an another image
• Click on the 'Open slide in new tab' menu option
• You can rearrange the images around by dragging the image header and move it around
• You can also arrange the slide-by-slide view by draggingb the lower image around
• Close one viewer window by clicking on the X in the image header
• Note: You can open multiple images (more than 2) side-by-side in the image viewer. There is hard limit on how many images you can open. However, the limit is practical based on your internet and laptop hardware

View IF slides

• From the slide list or from the main metadata table, open the slide where assay info starts with 'DAPI'
• Open the split channel view by clicking on 'Split channel' button
• Toggle color on by clicking on 'Toggle color' button
• Leave the split channel view by clicking on the 'Combine channels' button

Equilization settings

• Open the Equalization panel by clicking on it at the bottom right of the image viewer
• Select and unselect individual channels to only display channels you wish to visualize
• Change the color of a channel by clicking on the color box in front of it
• Change the value of the sliders for a channel

• Example: To remove the background stain, enter the value of 50 to the left limit for a channel

• Click on the drop-down arrow next to the 'Save this slide for me' button
• Click on the 'Save this slide for me' button

• Note: This will store the equalization panel settings for the current slide only for yourself

Create annotation set

To work with annotations you will need an annotation set selected.

• In the left-hand side panel, click on 'Annotations' tab
• Click on the 3 dots above annotation set drop-down field
• Click on the 'New...' option
• Enter a name for the annotation set
• Click on the 'Save' button

Rename annotation set

• In the left-hand side panel, click on 'Annotations' tab
• From the Annotation set drop-down field, select an existing annotation set
• Click on the 3 dots above annotation set drop-down field
• Click on the 'Rename...' option
• Enter a new name for the annotation set
• Click on the 'Save' button

Create region annotation

• Click on the polygon tool
• The 'Tumor area' class is preselected
• Draw an annotation by clicking and dragging left mouse button on the image
• Click on the 'Tumor area' floating panel at the bottom of the image viewer
• Select a new class by clicking on the class name from the annotation class selection panel
• Draw another annotation by clicking and dragging left mouse button on the image

Correct region annotation

• Hover over the annotation border
• Click and drag the border with a left mouse button to correct the annotation

Line annotation

• Start drawing a region annotation by click and dragging left mouse button on the image, without letting the button go
• Press the 'Shift' key on your keybaord
• Draw the line annotation
• Release the left mouse button
• Release the 'Shift' key

Magnet tool

• While the polygon (region annotation) tool is still active, click on the magnet tool
• Draw a polygon that is touching the polygon you have previously drawn
• Un-select the magnet tool when you are done drawing

Create FOV: Square

• Click on the FOV tool (square icon)
• Select the square
• Enter a desired value in the side length field, e.g. 100
• Pan around to find a good area with a few marker-positive cells
• Click on th eimage to place your FOV at the desired location

Create FOV: Square

• Click on the FOV tool (square icon)
• Select the circle
• Enter a desired value in the side length field, e.g. 100
• Pan around to find a good area with a few marker-positive cells
• Click on th eimage to place your FOV at the desired location

Transform region annotations

• Make sure all drawing tools are de-selected
• Click on the transform tool
• Select any existing region annotation on the image
• Grab the annotation and move it to a new location
• Make it bigger or rotate it

Re-classify region annotation

• Make sure all drawing tools are de-selected
• Click on the transform tool
• Select any existing region annotation on the image
• Grab the annotation and move it to a new location
• Make the annotation bigger by clicking and dragging on one of the edge-squares
• Rotate the annotation by clicking and dragging on the two sided edge-arrow

Delete annotation

• Delete an annotation using Annotations panel

• In the Annotations panel to the left, select annotations you wish to delete
• Click on the three dots
• Click on "Delete Annotations" option

• Delete an annotation using keyboard keys

• Make sure the selection tool is active
• Select any cell annotation
• Press the "Delete" key on your PC or the combination of keys "FN + Backspace" on your Mac

Create CMA: Rating

To work with annotations you will need an annotation set selected.

• Click on the cell marker annotations (CMA) tool (circular icon that looks like a target) in the Image viewer
• Click on the drop-down arrow at the gray top line of the CMA panel
• Click on the 'Rating' option
• Select a rating you wish to place: + (positive), - (negative), or ? (ambiguous)
• Select a the class you wish to place from the class list
• Click on the image in the Image viewer where you want to place a CMA with the selected class+rating

Create CMA: Intensity score

• Click on the cell marker annotations (CMA) tool (circular icon that looks like a target) in the Image viewer
• Click on the drop-down arrow at the gray top line of the CMA panel
• Click on the 'Intensity score' option
• Select a rating you wish to place: 0, 1+, 2+, or 3+
• Select a the class you wish to place from the class list
• Click on the image in the Image viewer where you want to place a CMA with the selected class+score

Add CMA class

• Click on the cell marker annotations (CMA) tool (circular icon that looks like a target) in the Image viewer
• Click on the "Add Class" button at the bottom of the CMA panel
• Click on the class you wish to add, e.g. Tumor

Add multiple CMAs

• Click on the cell marker annotations (CMA) tool (circular icon that looks like a target) in the Image viewer
• Click on the "Add Class" button at the bottom of the CMA panel
• Add at least two classes, e.g. Tumor and the slide's assay marker
• Select the Tumor class from the list
• Select the positive rating
• Click somewhere on the image to place a CMA
• Select the marker class (which marker is available depends on the value from the Assay info of that slide)
• Select the 1+ intensity score
• Click on top fo the existing CMA.

• Note: This will create one CMA with two scores: Tumor positive and Marker 1+

Delete CMA

• Select the same class of the CMA which is to be deleted
• Select any rating or intensity score
• Click on the CMA you wish to delete

• Note: A CMA can be deleted by using the same class of the CMA. Which rating or intensity score is selected is not relevant when deleting a CMA

Distance measurement

Distance measurements (ruler tool) work both with and without an annotation set selected.

• Select the ruler tool (ruler icon) in the image viewer
• Click somewhere on the slide where you want to start the distance measurement from
• Click somewhere on the slide where you want to end the distance measurement to
• Note: After placing a distance measurement you can click and drag one of the measurement points to adjust the distance measurement

Hide labels

• Clicking on the hide labels button (the T icon) at the bottom of the Image viewer will hide all labels from the Image viewer
• Clicking on the hide labels button for the second time will show all labels in the Image viewer
• Note: The state of the hide labels button is stored per session, when navigating between the slides

Hide all annotations

• Clicking on the hide annotations button (the eye icon) at the bottom of the Image viewer will hide all annotations from the Image viewer
• Clicking on the hide annotations button for the second time will show all annotations in the Image viewer

Move annotations

• Make sure that all annotation tools are unselected
• Click on the Move annotations tool (the double-sided arrow icon) at the bottom of the Image viewer
• Click on the Move annotations tool (the double-sided arrow icon) at the bottom of the Image viewer
• Click on an annotation in the Image viewer
• Click-and-drag an annotation to move it around in the Image viewer

Annotation sets overview page

• In the left-hand side panel, click on 'Annotations Sets' page
• Note: All annotation sets with the associated annotations will be shown for each slide

Sorting entries

Entries can be sorted only by slide information, and not by annotation counts.

• Clicking the first time on the header will sort the table in an ascending order
• Clicking the second time on the header will sort the table in a descending order
• Clicking the third time on the header will revert the table to its original state

Grouping entries

Entries can be grouped only by slide information, and not by annotation counts.

• Drag one fo the slide-related headers to the row above, where it says "Drag here to set row groups". This will group all table entries according to the selected column. By default, the table is grouped by the 'Filename' column
• You can drag multiple headers, one after the other. This will group all entries according to the selected columns in the correct order
• Clicking on the 'X' button next to the group name will ungroup the table by that column